Proteintech, 66308-1-Ig, CD27 Monoclonal antibody

Size: 20ul / 150ul The CD27 (66308-1-Ig) by Proteintech is a Monoclonal antibody targeting CD27 in WB, IHC, IF-P, FC, ELISA applications with reactivity to human, pig samples 66308-1-Ig targets CD27 in WB, IHC, IF-P, FC, ELISA applications and shows reactivity with human, pig samples. Tested Applications Positive WB detected in: Raji cells, pig spleen tissue, Ramos cells, Daudi cells Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human tonsillitis tissue Positive FC detected in: human PBMCs Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1. Specification Tested Reactivity: human, pig Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag24537 Product name: Recombinant human CD27 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-260 aa of BC012160 Sequence: MARPHPWWLCVLGTLVGLSATPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTECDPLPNPSLTARSSQALSPHPQPTHLPYVSEMLEARTAGHMQTLADFRQLPARTLSTHWPPQRSLCSSDFIRILVIFSGMFLVFTLAGALFLHQRRKYRSNKGESPVEPAEPCRYSCPREEEGSTIPIQEDYRKPEPACSP Predict reactive species Full Name: CD27 molecule Calculated Molecular Weight: 29 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC012160 Gene Symbol: CD27 Gene ID (NCBI): 939 ENSEMBL Gene ID: ENSG00000139193 RRID: AB_2881690 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P26842 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.