Proteintech, 66311-1-Ig, HEXIM1 Monoclonal antibody

Size: 20ul / 150ul The HEXIM1 (66311-1-Ig) by Proteintech is a Monoclonal antibody targeting HEXIM1 in WB, ELISA applications with reactivity to human, mouse, rat samples 66311-1-Ig targets HEXIM1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MDA-MB-231 cells, Caco-2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgM Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8019 Product name: Recombinant human HEXIM1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-359 aa of BC006460 Sequence: MAEPFLSEYQHQPQTSNCTGAAAVQEELNPERPPGAEERVPEEDSRWQSRAFPQLGGRPGPEGEGSLESQPPPLQTQACPESSCLREGEKGQNGDDSSAGGDFPPPAEVEPTPEAELLAQPCHDSEASKLGAPAAGGEEEWGQQQRQLGKKKHRRRPSKKKRHWKPYYKLTWEEKKKFDEKQSLRASRIRAEMFAKGQPVAPYNTTQFLMDDHDQEEPDLKTGLYSKRAAAKSDDTSDDDFMEEGGEEDGGSDGMGGDGSEFLQRDFSETYERYHTESLQNMSKQELIKEYLELEKCLSRMEDENNRLRLESKRLGGDDARVRELELELDRLRAENLQLLTENELHRQQERAPLSKFGD Predict reactive species Full Name: hexamethylene bis-acetamide inducible 1 Calculated Molecular Weight: 41 kDa Observed Molecular Weight: 54 kDa GenBank Accession Number: BC006460 Gene Symbol: HEXIM1 Gene ID (NCBI): 10614 RRID: AB_2881693 Conjugate: Unconjugated Form: Liquid Purification Method: Thiophilic affinity chromatograph UNIPROT ID: O94992 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.