Proteintech, 66317-1-Ig, Peripherin Monoclonal antibody

Size: 20ul / 150ul The Peripherin (66317-1-Ig) by Proteintech is a Monoclonal antibody targeting Peripherin in WB, IHC, FC (Intra), ELISA applications with reactivity to human, rat, pig samples 66317-1-Ig targets Peripherin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, rat, pig samples. Tested Applications Positive WB detected in: IMR-32 cells, human colon tissue, rat brain tissue, rat colon tissue, mouse colon tissue, rat spinal cord tissue Positive IHC detected in: human brain tissue, human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: PC-12 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Peripherin is a neuron-specific intermediate filament protein that is particularly expressed in peripheral nervous system. It is used to study peripheral nerve development and regeneration, as it is highly expressed during elongation of axon. Peripherin is a routine marker for unmyelinated neurons in trigeminal ganglion (TG) and the dorsal root ganglion (DRG). Peripherin is also expressed in peri-insular areas of islets and it has been identified as a key autoantigen for type 1A diabetes. Specification Tested Reactivity: human, rat, pig Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag11116 Product name: Recombinant human Peripherin protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 155-470 aa of BC032703 Sequence: LELLGRERDRVQVERDGLAEDLAALKQRLEEETRKREDAEHNLVLFRKDVDDATLSRLELERKIESLMDEIEFLKKLHEEELRDLQVSVESQQVQQVEVEATVKPELTAALRDIRAQYESIAAKNLQEAEEWYKSKYADLSDAANRNHEALRQAKQEMNESRRQIQSLTCEVDGLRGTNEALLRQLRELEEQFALEAGGYQAGAARLEEELRQLKEEMARHLREYQELLNVKMALDIEIATYRKLLEGEESRISVPVHSFASLNIKTTVPEVEPPQDSHSRKTVLIKTIETRNGEVVTESQKEQRSELDKSSAHSY Predict reactive species Full Name: peripherin Calculated Molecular Weight: 470 aa, 54 kDa Observed Molecular Weight: 50-54 kDa GenBank Accession Number: BC032703 Gene Symbol: Peripherin Gene ID (NCBI): 5630 RRID: AB_2881698 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P41219 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.