Proteintech, 66325-1-Ig, Factor XIIIa Monoclonal antibody

Size: 20ul / 150ul The Factor XIIIa (66325-1-Ig) by Proteintech is a Monoclonal antibody targeting Factor XIIIa in WB, IHC, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 66325-1-Ig targets Factor XIIIa in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: pig colon tissue, human placenta tissue, human peripheral blood platelets, rat colon tissue, rabbit large intestine tissue, pig liver tissue Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: U-937 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:100-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Factor XIIIa is a blood proenzyme that catalyzes intermolecular cross-linking of fibrinogen and is involved in clotting cascade. It has been identified in various tissues including placenta, uterus, and prostate. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (90% positive) and desmoplastic malignant melanoma (negative). Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor , and meningioma. Specification Tested Reactivity: human, mouse, rat, pig, rabbit Cited Reactivity: human Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag11105 Product name: Recombinant human F13A1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 383-732 aa of BC027963 Sequence: RPDLPVGFGGWQAVDSTPQENSDGMYRCGPASVQAIKHGHVCFQFDAPFVFAEVNSDLIYITAKKDGTHVVENVDATHIGKLIVTKQIGGDGMMDITDTYKFQEGQEEERLALETALMYGAKKPLNTEGVMKSRSNVDMDFEVENAVLGKDFKLSITFRNNSHNRYTITAYLSANITFYTGVPKAEFKKETFDVTLEPLSFKKEAVLIQAGEYMGQLLEQASLHFFVTARINETRDVLAKQKSTVLTIPEIIIKVRGTQVVGSDMTVIVEFTNPLKETLRNVWVHLDGPGVTRPMKKMFREIRPNSTVQWEEVCRPWVSGHRKLIASMSSDSLRHVYGELDVQIQRRPSM Predict reactive species Full Name: coagulation factor XIII, A1 polypeptide Calculated Molecular Weight: 732 aa, 83 kDa Observed Molecular Weight: 75-80 kDa GenBank Accession Number: BC027963 Gene Symbol: Factor XIIIa Gene ID (NCBI): 2162 RRID: AB_2881706 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P00488 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.