Proteintech, 66342-1-Ig, PLOD2 Monoclonal antibody

Size: 20ul / 150ul The PLOD2 (66342-1-Ig) by Proteintech is a Monoclonal antibody targeting PLOD2 in WB, IHC, ELISA applications with reactivity to human samples 66342-1-Ig targets PLOD2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: DU 145 cells, A431 cells, HEK-293 cells, THLE-2 cells, A549 cells, Hela cells, HEK293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:200-1:1000 Background Information PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy.(PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgA Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag5779 Product name: Recombinant human PLOD2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 410-758 aa of BC037169 Sequence: NRKIIAPLVTRHGKLWSNFWGALSPDGYYARSEDYVDIVQGNRVGVWNVPYMANVYLIKGKTLRSEMNERNYFVRDKLDPDMALCRNAREMTLQREKDSPTPETFQMLSPPKGVFMYISNRHEFGRLLSTANYNTSHYNNDLWQIFENPVDWKEKYINRDYSKIFTENIVEQPCPDVFWFPIFSEKACDELVEEMEHYGKWSGGKHHDSRISGGYENVPTDDIHMKQVDLENVWLHFIREFIAPVTLKVFAGYYTKGFALLNFVVKYSPERQRSLRPHHDASTFTINIALNNVGEDFQGGGCKFLRYNCSIESPRKGWSFMHPGRLTHLHEGLPVKNGTRYIAVSFIDP Predict reactive species Full Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 Calculated Molecular Weight: 758 aa, 85 kDa Observed Molecular Weight: 87 kDa GenBank Accession Number: BC037169 Gene Symbol: PLOD2 Gene ID (NCBI): 5352 RRID: AB_2881722 Conjugate: Unconjugated Form: Liquid Purification Method: Thiophilic affinity chromatograph UNIPROT ID: O00469 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.