Proteintech, 66353-1-Ig, CBLB Monoclonal antibody

Size: 20ul / 150ul The CBLB (66353-1-Ig) by Proteintech is a Monoclonal antibody targeting CBLB in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, pig, rat samples 66353-1-Ig targets CBLB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig, rat samples. Tested Applications Positive WB detected in: RAW 264.7 cells, rat heart tissue Positive IHC detected in: human heart tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:100-1:400 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CBLB(E3 ubiquitin-protein ligase CBL-B) is also known as RNF56 and plays a necessary role to prevent the inappropriate activation of NF-B in response to lipopolysaccharide and to polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.. It also has a crucial role in the prevention of chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing. Specification Tested Reactivity: human, mouse, pig, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG3 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag18757 Product name: Recombinant human CBLB protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-352 aa of BC032851 Sequence: MANSMNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKNSPPYILDILPDTYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDRRNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKYSTKPGSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDGRSYNPDLTGLCEPTPHDH Predict reactive species Full Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence b Calculated Molecular Weight: 982 aa, 109 kDa Observed Molecular Weight: 110 kDa GenBank Accession Number: BC032851 Gene Symbol: CBLB Gene ID (NCBI): 868 RRID: AB_2881733 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13191 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.