Proteintech, 66360-3-Ig, Mouse IgG2b isotype control Monoclonal antibody

Size: 150ul The Mouse IgG2b isotype control (66360-3-Ig) by Proteintech is a Monoclonal antibody targeting Mouse IgG2b isotype control in WB, IHC, FC, ELISA applications with reactivity to megathura crenulata samples 66360-3-Ig targets Mouse IgG2b isotype control in WB, IHC, FC, IP, chIP, ELISA applications and shows reactivity with megathura crenulata samples. Tested Applications Positive IHC detected in: Note: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC detected in: human PBMCs Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:1500-1:6000 Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension Background Information This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH). Specification Tested Reactivity: megathura crenulata Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: Mouse IgG2b isotype control RRID: AB_2881740 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.