Proteintech, 66369-1-Ig, PGC1a Monoclonal antibody

Size: 20ul / 150ul The PGC1a (66369-1-Ig) by Proteintech is a Monoclonal antibody targeting PGC1a in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 66369-1-Ig targets PGC1a in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: pig skeletal muscle tissue, HSC-T6 cells, rat skeletal muscle tissue, mouse skeletal muscle tissue Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 Background Information PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1. Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse, rat, pig, monkey, chicken, zebrafish, hamster, goat, ducks Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: peroxisome proliferator-activated receptor gamma, coactivator 1 alpha Calculated Molecular Weight: 91 kDa Observed Molecular Weight: 70 kDa, 100 kDa GenBank Accession Number: NM_013261 Gene Symbol: PGC1a Gene ID (NCBI): 10891 RRID: AB_2828002 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9UBK2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.