Proteintech, 66416-1-Ig, P2RX4 Monoclonal antibody

Size: 20ul / 150ul The P2RX4 (66416-1-Ig) by Proteintech is a Monoclonal antibody targeting P2RX4 in WB, IHC, ELISA applications with reactivity to human, mouse, pig, rat samples 66416-1-Ig targets P2RX4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig, rat samples. Tested Applications Positive WB detected in: HeLa cells, A549 cells, mouse brain tissue, rat brain tissue, rabbit brain tissue, Neuro-2a cells, human brain tissue, pig brain tissue Positive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RX4 (P2X purinoceptor 4) is a receptor for ATP acting as a ligand gated ion channel. The calculated molecular weight of P2RX4 is 43 kDa, larger apparent molecular weights of 50-80 kDa have been reported, possibly due to post-translational glycosylation (PMID: 24040145, 15262999; 29382907; 12566439). Specification Tested Reactivity: human, mouse, pig, rat Cited Reactivity: mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag4445 Product name: Recombinant human P2RX4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 57-341 aa of BC033826 Sequence: TDSVVSSVTTKVKGVAVTNTSKLGFRIWDVADYVIPAQEENSLFVMTNVILTMNQTQGLCPEIPDATTVCKSDASCTAGSAGTHSNGVSTGRCVAFNGSVKTCEVAAWCPVEDDTHVPQPAFLKAAENFTLLVKNNIWYPKFNFSKRNILPNITTTYLKSCIYDAKTDPFCPIFRLGKIVENAGHGFQDMAVEGGIMGIQVNWDCNLDRAASLCLPRYSFRRLDTRDVEHNVSPGYNFRFAKYYRDLAGNEQRTLIKAYGIRFDIIVFGKAGKFDIIPTMINIGS Predict reactive species Full Name: purinergic receptor P2X, ligand-gated ion channel, 4 Calculated Molecular Weight: 388 aa, 43 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC033826 Gene Symbol: P2RX4 Gene ID (NCBI): 5025 RRID: AB_2881788 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q99571 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.