Proteintech, 66420-1-Ig, CDC37 Monoclonal antibody

Size: 20ul / 150ul The CDC37 (66420-1-Ig) by Proteintech is a Monoclonal antibody targeting CDC37 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 66420-1-Ig targets CDC37 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, ROS1728 cells, RAW 264.7 cells, NIH/3T3 cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:5000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CDC(cell division control) family of proteins are regulatory proteins involved in the processes of cell cycle and proliferation. CDC37, migrated as a protein of 50 kDa in SDS PAGE, is a co-chaperone protein that recruits several immature client kinases to Hsp90 for proper folding. It functions in association with Hsp90, although little is known of its mechanism of action or where it fits into a folding pathway involving other Hsp90 cochaperones. CDC37 up-regulation is a common early event in some localized human cancers. But, the effect of CDC37 dysregulation in human cells is unclear. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag0267 Product name: Recombinant human CDC37 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-209 aa of BC000083 Sequence: MVDYSVWDHIEVSDDEDETHPNIDTASLFRWRHQARVERMEQFQKEKEELDRGCRECKRKVAECQRKLKELEVAEGGKAELERLQAEAQQLRKEERSWEQKLEEMRKKEKSMPWNVDTLSKDGFSKSMVNTKPEKTEEDSEEVREQKHKTFVEKYEKQIKHFGMLRRWDDSQKYLSDNVHLVCEETANYLVIWCIDLEVEEKCALMEQV Predict reactive species Full Name: cell division cycle 37 homolog (S. cerevisiae) Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 44 kDa GenBank Accession Number: BC000083 Gene Symbol: CDC37 Gene ID (NCBI): 11140 RRID: AB_2881792 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q16543 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.