Proteintech, 66497-1-Ig, CCAR2 Monoclonal antibody

Size: 20ul / 150ul The CCAR2 (66497-1-Ig) by Proteintech is a Monoclonal antibody targeting CCAR2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, pig samples 66497-1-Ig targets CCAR2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, pig samples. Tested Applications Positive WB detected in: HeLa cells, pig brain tissue, HEK-293 cells, MCF-7 cells, Jurkat cells Positive IHC detected in: human cervical cancer tissue, human colon tissue, human lung cancer tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information Cell cycle and apoptosis regulator protein 2 (CCAR2), previously known as DBC1/KIAA1967, regulates diverse cellular functions including transcription, mRNA splicing, metabolism, and circadian cycle. CCAR2 is a critical regulator of cell death or survival following cellular stresses. CCAR2 is a widely expressed protein involved in the regulation of a variety of transcriptional complexes. High expression of CCAR2 correlates with poor outcomes in many human tumor types such as squamous cell carcinoma (SCC). Specification Tested Reactivity: human, pig Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag18374 Product name: Recombinant human DBC1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 571-923 aa of BC018269 Sequence: EPEKEEAAKEEATKEEEAIKEEVVKEPKDEAQNEGPATESEAPLKEDGLLPKPLSSGGEEEEKPRGEASEDLCEMALDPELLLLRDDGEEEFAGAKLEDSEVRSVASNQSEMEFSSLQDMPKELDPSAVLPLDCLLAFVFFDANWCGYLHRRDLERILLTLGIRLSAEQAKQLVSRVVTQNICQYRSLQYSRQEGLDGGLPEEVLFGNLDLLPPPGKSTKPGAAPTEHKALVSHNGSLINVGSLLQRAEQQDSGRLYLENKIHTLELKLEESHNRFSATEVTNKTLAAEMQELRVRLAEAEETARTAERQKSQLQRLLQELRRRLTPLQLEIQRVVEKADSWVEKEEPAPSN Predict reactive species Full Name: KIAA1967 Calculated Molecular Weight: 923 aa, 103 kDa Observed Molecular Weight: 130 kDa GenBank Accession Number: BC018269 Gene Symbol: CCAR2 Gene ID (NCBI): 57805 RRID: AB_2881861 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q8N163 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.