Proteintech, 66502-1-Ig, CD40L/CD154 Monoclonal antibody

Size: 20ul / 150ul The CD40L/CD154 (66502-1-Ig) by Proteintech is a Monoclonal antibody targeting CD40L/CD154 in WB, ELISA applications with reactivity to Human, rat samples 66502-1-Ig targets CD40L/CD154 in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat samples. Tested Applications Positive WB detected in: Jurkat cells, human peripheral blood platelets Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1. Specification Tested Reactivity: Human, rat Cited Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag24941 Product name: Recombinant human CD40L; CD154 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 47-261 aa of BC071754 Sequence: HRRLDKIEDERNLHEDFVFMKTIQRCNTGERSLSLLNCEEIKSQFEGFVKDIMLNKEETKKENSFEMQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL Predict reactive species Full Name: CD40 ligand Calculated Molecular Weight: 261 aa, 29 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: BC071754 Gene Symbol: CD40 Ligand Gene ID (NCBI): 959 RRID: AB_2881866 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P29965 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.