Proteintech, 66506-1-Ig, MYPT1 Monoclonal antibody

Size: 20ul / 150ul The MYPT1 (66506-1-Ig) by Proteintech is a Monoclonal antibody targeting MYPT1 in WB, IF/ICC, ELISA applications with reactivity to Human, rat, mouse samples 66506-1-Ig targets MYPT1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples. Tested Applications Positive WB detected in: Raji cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization. Specification Tested Reactivity: Human, rat, mouse Cited Reactivity: human, rat Host / Isotype: Mouse / IgG3 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17496 Product name: Recombinant human MYPT1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 707-981 aa of BC111752 Sequence: AEKTIGRSRSTRTREQENEEKEKEEKEKQDKEKQEEKKESETSREDEYKQKYSRTYDETYQRYRPVSTSSSTTPSSSLSTMSSSLYASSQLNRPNSLVGITSAYSRGITKENEREGEKREEEKEGEDKSQPKSIRERRRPREKRRSTGVSFWTQDSDENEQEQQSDTEEGSNKKETQTDSISRYETSSTSAGDRYDSLLGRSGSYSYLEERKPYSSRLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKATQRQERFADRSLLE Predict reactive species Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 12A Calculated Molecular Weight: 1030 aa, 115 kDa Observed Molecular Weight: 115 kDa GenBank Accession Number: BC111752 Gene Symbol: MYPT1 Gene ID (NCBI): 4659 RRID: AB_2881869 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O14974 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.