Proteintech, 66548-1-Ig, FMRP Monoclonal antibody

Size: 20ul / 150ul The FMRP (66548-1-Ig) by Proteintech is a Monoclonal antibody targeting FMRP in WB, IHC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 66548-1-Ig targets FMRP in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, HSCT6 cells Positive IP detected in: HeLa cells Positive IHC detected in: human gliomas tissue, mouse brain tissue, human liver tissue, human colon cancer tissue, human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: Jurkat cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag4697 Product name: Recombinant human FMR1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-297 aa of BC038998 Sequence: MEELVVEVRGSNGAFYKAFVKDVHEDSITVAFENNWQPDRQIPFHDVRFPPPVGYNKDINESDEVEVYSRANEKEPCCWWLAKVRMIKGEFYVIEYAACDATYNEIVTIERLRSVNPNKPATKDTFHKIKLDVPEDLRQMCAKEAAHKDFKKAVGAFSVTYDPENYQLVILSINEVTSKRAHMLIDMHFRSLRTKLSLIMRNEEASKQLESSRQLASRFHEQFIVREDLMGLAIGTHGANIQQARKVPGVTAIDLDEDTCTFHIYGEDQDAVKKARSFLEFAEDVIQVPRNLVGLKI Predict reactive species Full Name: fragile X mental retardation 1 Calculated Molecular Weight: 632 aa, 71 kDa Observed Molecular Weight: 69-72 kDa GenBank Accession Number: BC038998 Gene Symbol: FMR1 Gene ID (NCBI): 2332 RRID: AB_2881910 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q06787 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.