Proteintech, 66556-1-Ig, BCL10 Monoclonal antibody

Size: 20ul / 150ul The BCL10 (66556-1-Ig) by Proteintech is a Monoclonal antibody targeting BCL10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 66556-1-Ig targets BCL10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, Raji cells, HepG2 cells, HCT 116 cells, MOLT-4 cells, Jurkat cells, K-562 cells, Ramos cells Positive IHC detected in: human tonsillitis tissue, human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: Jurkat cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:350-1:1400 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag12162 Product name: Recombinant human BCL10 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-233 aa of BC053617 Sequence: MEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTEEISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQKITDEVLKLRNIKLEHLKGLKCSSCEPFPDGATNNLSRSNSDESNFSEKLRASTVMYHPEGESSTTPFFSTNSSLNLPVLEVGRTENTIFSSTTLPRPGDPGAPPLPPDLQLEEEGTCANSSEMFLPLRSRTVSRQ Predict reactive species Full Name: B-cell CLL/lymphoma 10 Calculated Molecular Weight: 233 aa, 26 kDa Observed Molecular Weight: 30-32 kDa GenBank Accession Number: BC053617 Gene Symbol: BCL10 Gene ID (NCBI): 8915 RRID: AB_2881917 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O95999 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.