Proteintech, 66570-1-Ig, TLR5 Monoclonal antibody

Size: 20ul / 150ul The TLR5 (66570-1-Ig) by Proteintech is a Monoclonal antibody targeting TLR5 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat, pig samples 66570-1-Ig targets TLR5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: A549 cells, THP-1 cells, rat spleen tissue, HeLa cells, RAW 264.7 cells Positive IF/ICC detected in: THP-1 cells Positive FC (Intra) detected in: THP-1 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information TLR5 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR5 is known to recognize bacterial flagellin from invading mobile bacteria. TLR5 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Mutations in TLR5 gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease. Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13840 Product name: Recombinant human TLR5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 661-858 aa of BC109119 Sequence: TKFRGFCFICYKTAQRLVFKDHPQGTEPDMYKYDAYLCFSSKDFTWVQNALLKHLDTQYSDQNRFNLCFEERDFVPGENRIANIQDAIWNSRKIVCLVSRHFLRDGWCLEAFSYAQGRCLSDLNSALIMVVVGSLSQYQLMKHQSIRGFVQKQQYLRWPEDLQDVGWFLHKLSQQILKKEKEKKKDNNIPLQTVATIS Predict reactive species Full Name: toll-like receptor 5 Calculated Molecular Weight: 858 aa, 98 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC109119 Gene Symbol: TLR5 Gene ID (NCBI): 7100 RRID: AB_2881931 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O60602 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.