Proteintech, 66594-1-Ig, MAdCAM1 Monoclonal antibody

Size: 20ul / 150ul The MAdCAM1 (66594-1-Ig) by Proteintech is a Monoclonal antibody targeting MAdCAM1 in IHC, IF-P, ELISA applications with reactivity to Human samples 66594-1-Ig targets MAdCAM1 in IHC, IF-P, ELISA applications and shows reactivity with Human samples. Tested Applications Positive IHC detected in: human colon tissue, human tonsillitis tissue, human appendicitis tissue, human small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human tonsillitis tissue Recommended dilution Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Specification Tested Reactivity: Human Cited Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag16184 Product name: Recombinant human MADCAM1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 24-343 aa of BC142629 Sequence: KPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLGAVQSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPDQLTVSPAALVPGDPEVACTAHKVTPVDPNALSFSLLVGGQELEGAQALGPEVQEEEEEPQGDEDVLFRVTERWRLPPLGTPVPPALYCQATMRLPGLELSHRQAIPVLHSPTSPEPPDTTSPEPPNTTSPESPDTTSPESPDTTSQEPPDTTSQEPPDTTSQEPPDTTSPEPPDKTSPEPAPQQGSTHTPRSPGSTRTRRPEISQAGPTQGEVIPTGSSKPAGDQLP Predict reactive species Full Name: mucosal vascular addressin cell adhesion molecule 1 Calculated Molecular Weight: 406 aa, 43 kDa GenBank Accession Number: BC142629 Gene Symbol: MADCAM1 Gene ID (NCBI): 8174 RRID: AB_2881954 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13477 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.