Proteintech, 66606-1-Ig, FYN Monoclonal antibody

Size: 20ul / 150ul The FYN (66606-1-Ig) by Proteintech is a Monoclonal antibody targeting FYN in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 66606-1-Ig targets FYN in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, HSC-T6 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, Ramos cells, PC-12 cells, 4T1 cells Positive IHC detected in: human tonsillitis tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Neuro-2a cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:30000 Immunohistochemistry (IHC): IHC : 1:2000-1:5000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information FYN, also named p59-Fyn and SLK, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. FYN is implicated in the control of cell growth. It plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. FYN is required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. FYN blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag11127 Product name: Recombinant human FYN protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 231-483 aa of BC032496 Sequence: YSGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQSFLEDYFTATEPQYQPGENL Predict reactive species Full Name: FYN oncogene related to SRC, FGR, YES Calculated Molecular Weight: 537aa,61 kDa; 482aa,55 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC032496 Gene Symbol: FYN Gene ID (NCBI): 2534 RRID: AB_2881966 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P06241 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.