Proteintech, 66617-1-Ig, ACSL4/FACL4 Monoclonal antibody

Size: 20ul / 150ul The ACSL4/FACL4 (66617-1-Ig) by Proteintech is a Monoclonal antibody targeting ACSL4/FACL4 in WB, IHC, FC (Intra), ELISA applications with reactivity to human samples 66617-1-Ig targets ACSL4/FACL4 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, Caco-2 cells, COLO 320 cells, L02 cells, HT-29 cells Positive IHC detected in: human cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:3000-1:10000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Acyl-CoA synthetase long-chain family 4 (ACSL4) is a key enzyme that catalyzes long-chain fatty acids activation, and abnormal expression of ACSL4 is closely related to various biological responses, including steroidogenesis, inflammation response, cell death, immune activation response, and so on. Specifically, ACSL4 participates in ferroptosis, a promising target for tumor therapeutics. Specification Tested Reactivity: human Cited Reactivity: human, rat, bovine, sheep Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag18085 Product name: Recombinant human ACSL4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 143-236 aa of BC034959 Sequence: LGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMY Predict reactive species Full Name: acyl-CoA synthetase long-chain family member 4 Calculated Molecular Weight: 711 aa, 79 kDa Observed Molecular Weight: 74 kDa GenBank Accession Number: BC034959 Gene Symbol: ACSL4 Gene ID (NCBI): 2182 RRID: AB_2881977 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O60488 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.