Proteintech, 66625-1-Ig, RIC8A Monoclonal antibody

Size: 20ul / 150ul The RIC8A (66625-1-Ig) by Proteintech is a Monoclonal antibody targeting RIC8A in WB, IHC, IF-P, IP, ELISA applications with reactivity to Human, Rat, Mouse samples 66625-1-Ig targets RIC8A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with Human, Rat, Mouse samples. Tested Applications Positive WB detected in: HeLa cells, A431 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, RAW 264.7 cells, NIH/3T3 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: mouse brain tissue, human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation. Specification Tested Reactivity: Human, Rat, Mouse Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag1593 Product name: Recombinant human RIC8A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 117-467 aa of BC011821 Sequence: QQLFQELKGVRLLTDTLELTLGVTPEGNPPTLLPSQETERAMEILKVLFNITLDSIKGEVDEEDAALYRHLGTLLRHCVMIATAGDRTEEFHGHAVNLLGNLPLKCLDVLLTLEPHGDSTEFMGVNMDVIRALLIFLEKRLHKTHRLKESVAPVLSVLTECARMHRPARKFLKAQVLPPLRDVRTRPEVGEMLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLAARGLMAGGRPEGQYSEDEDTDTDEYKEAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAMKLVTMFDKLSRNRVIQPMGMSPRGHLTSLQDAMCETMEQQLSSDPDSDPD Predict reactive species Full Name: resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) Calculated Molecular Weight: 60 kDa Observed Molecular Weight: 65 kDa GenBank Accession Number: BC011821 Gene Symbol: RIC8A Gene ID (NCBI): 60626 RRID: AB_2881985 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9NPQ8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.