Proteintech, 66646-1-Ig, EIF2AK2, PKR Monoclonal antibody

Size: 20ul / 150ul The EIF2AK2, PKR (66646-1-Ig) by Proteintech is a Monoclonal antibody targeting EIF2AK2, PKR in WB, IHC, ELISA applications with reactivity to human samples 66646-1-Ig targets EIF2AK2, PKR in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells Positive IHC detected in: human testis tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13010 Product name: Recombinant human EIF2AK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-373 aa of BC093676 Sequence: MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGT Predict reactive species Full Name: eukaryotic translation initiation factor 2-alpha kinase 2 Calculated Molecular Weight: 551 aa, 62 kDa Observed Molecular Weight: 65-68 kDa GenBank Accession Number: BC093676 Gene Symbol: EIF2AK2 Gene ID (NCBI): 5610 RRID: AB_2882004 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P19525 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.