Proteintech, 66652-1-Ig, XRCC2 Monoclonal antibody

Size: 20ul / 150ul The XRCC2 (66652-1-Ig) by Proteintech is a Monoclonal antibody targeting XRCC2 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 66652-1-Ig targets XRCC2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, Raji cells, NIH/3T3 cells Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information XRCC2, also named as DNA repair protein XRCC2, is a 280 amino acid protein, which belongs to the RecA family. XRCC2 is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. XRCC2 is a part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Specification Tested Reactivity: human, mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag14421 Product name: Recombinant human XRCC2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-280 aa of BC042137 Sequence: MCSAFHRAESGTELLARLEGRSSLKEIEPNLFADEDSPVHGDILEFHGPEGTGKTEMLYHLTARCILPKSEGGLEVEVLFIDTDYHFDMLRLVTILEHRLSQSSEEIIKYCLGRFFLVYCSSSTHLLLTLYSLESMFCSHPSLCLLILDSLSAFYWIDRVNGGESVNLQESTLRKCSQCLEKLVNDYRLVLFATTQTIMQKASSSSEEPSHASRRLCDVDIDYRPYLCKAWQQLVKHRMFFSKQDDSQSSNQFSLVSRCLKSNSLKKHFFIIGESGVEFC Predict reactive species Full Name: X-ray repair complementing defective repair in Chinese hamster cells 2 Calculated Molecular Weight: 280 aa, 32 kDa Observed Molecular Weight: 32 kDa GenBank Accession Number: BC042137 Gene Symbol: XRCC2 Gene ID (NCBI): 7516 RRID: AB_2882009 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O43543 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.