Proteintech, 66711-1-Ig, B3GAT1 Monoclonal antibody

Size: 20ul / 150ul The B3GAT1 (66711-1-Ig) by Proteintech is a Monoclonal antibody targeting B3GAT1 in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 66711-1-Ig targets B3GAT1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: K-562 cells, mouse brain tissue, Jurkat cells, PC-3 cells, SH-SY5Y cells, U-251 cells Positive FC (Intra) detected in: K-562 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 66711-1-Ig antibody detectes 100 kDa form in SDS-PAGE which might as a result of homodimer or glycosylation. Specification Tested Reactivity: human, mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13593 Product name: Recombinant human CD57 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 25-334 aa of BC010466 Sequence: HQSTLAPLLAVHKDEGSDPRRETPPGADPREYCTSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI Predict reactive species Full Name: beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) Calculated Molecular Weight: 38 kDa Observed Molecular Weight: 38 kDa, 100 kDa GenBank Accession Number: BC010466 Gene Symbol: B3GAT1 Gene ID (NCBI): 27087 RRID: AB_2882062 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9P2W7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.