Proteintech, 66714-1-Ig, RHEB Monoclonal antibody

Size: 20ul / 150ul The RHEB (66714-1-Ig) by Proteintech is a Monoclonal antibody targeting RHEB in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse samples 66714-1-Ig targets RHEB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: fetal human brain tissue, HeLa cells, DU 145 cells, mouse brain tissue Positive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human pancreas cancer tissue, mouse pancreas tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:3000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8887 Product name: Recombinant human RHEB protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-184 aa of BC016155 Sequence: MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM Predict reactive species Full Name: Ras homolog enriched in brain Calculated Molecular Weight: 184 aa, 20 kDa Observed Molecular Weight: 18 kDa GenBank Accession Number: BC016155 Gene Symbol: RHEB Gene ID (NCBI): 6009 RRID: AB_2882065 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q15382 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.