Proteintech, 66721-1-Ig, SYK Monoclonal antibody

Size: 20ul / 150ul The SYK (66721-1-Ig) by Proteintech is a Monoclonal antibody targeting SYK in WB, IHC, IF-P, IP, ELISA applications with reactivity to human samples 66721-1-Ig targets SYK in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Raji cells, THP-1 cells, Ramos cells, Daudi cells Positive IP detected in: MDA-MB-231 cells Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human tonsillitis tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660) Specification Tested Reactivity: human Cited Reactivity: human, mouse, pig Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17654 Product name: Recombinant human SYK protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 239-427 aa of BC001645 Sequence: QLVEHYSYKADGLLRVLTVPCQKIGTQGNVNFGGRPQLPGSHPATWSAGGIISRIKSYSFPKPGHRKSSPAQGNRQESTVSFNPYEPELAPWAADKGPQREALPMDTEVYESPYADPEEIRPKEVYLDRKLLTLEDKELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAEANVMQQL Predict reactive species Full Name: spleen tyrosine kinase Calculated Molecular Weight: 635 aa, 72 kDa Observed Molecular Weight: 70-75 kDa GenBank Accession Number: BC001645 Gene Symbol: SYK Gene ID (NCBI): 6850 ENSEMBL Gene ID: ENSG00000165025 RRID: AB_2882072 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P43405 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.