Proteintech, 66739-1-Ig, ARL13B Monoclonal antibody

Size: 20ul / 150ul The ARL13B (66739-1-Ig) by Proteintech is a Monoclonal antibody targeting ARL13B in WB, IF/ICC, IF-Fro, ELISA applications with reactivity to human, mouse, pig, canine samples 66739-1-Ig targets ARL13B in WB, IHC, IF/ICC, IF-Fro, ELISA applications and shows reactivity with human, mouse, pig, canine samples. Tested Applications Positive WB detected in: HepG2 cells, HEK-293 cells, NCCIT cell Positive IF-Fro detected in: mouse heart tissue Positive IF/ICC detected in: hTERT-RPE1 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986). Specification Tested Reactivity: human, mouse, pig, canine Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag12031 Product name: Recombinant human ARL13B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-321 aa of BC094725 Sequence: MFSLMASCCGWFKRWREPVRLANKQDKEGALGEADVIECLSLEKLVNEHKCLCQIEPCSAISGYGKKIDKSIKKGLYWLLHVIARDFDALNERIQKETTEQRALEEQEKQERAERVRKLREERKQNEQEQAELDGTSGLAELDPEPTNPFQPIASVIIENEGKLEREKKNQKMEKDSDGCHLKHKMEHEQIETQGQVNHNGQKNNEFGLVENYKEALTQQLKNEDETDRPSLESANGKKKTKKLRMKRNHRVEPLNIDDCAPESPTPPPPPPPVGWGTPKVTRLPKLEPLGETHHNDFYRKPLPPLAVPQRPNSDAHDVIS Predict reactive species Full Name: ADP-ribosylation factor-like 13B Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC094725 Gene Symbol: ARL13B Gene ID (NCBI): 200894 RRID: AB_2882088 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q3SXY8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.