Proteintech, 66762-1-Ig, BACH1 Monoclonal antibody

Size: 20ul / 150ul The BACH1 (66762-1-Ig) by Proteintech is a Monoclonal antibody targeting BACH1 in WB, IHC, ELISA applications with reactivity to human, rat samples 66762-1-Ig targets BACH1 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, HSC-T6 cells, Jurkat cells, K-562 cells, Raji cells Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa. Specification Tested Reactivity: human, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6011 Product name: Recombinant human BACH1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 387-736 aa of BC063307 Sequence: SSVEREVAEHLAKGFWSDICSTDTPCQMQLSPAVAKDGSEQISQKRSECPWLGIRISESPEPGQRTFTTLSSVNCPFISTLSTEGCSSNLEIGNDDYVSEPQQEPCPYACVISLGDDSETDTEGDSESCSAREQECEVKLPFNAQRIISLSRNDFQSLLKMHKLTPEQLDCIHDIRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLQSEKESLLKERDHILSTLGETKQNLTGLCQKVCKEAALSQEQIQILAKYSAADCPLSFLISEKDKSTPDGELALPSIFSLSDRPPAVLPPCARGNSEPGYARGQESQQMSTATSEQAGPAEQCRQSGGISDFCQQMTDKCTTDE Predict reactive species Full Name: BTB and CNC homology 1, basic leucine zipper transcription factor 1 Calculated Molecular Weight: 82 kDa Observed Molecular Weight: 100-110 kDa GenBank Accession Number: BC063307 Gene Symbol: BACH1 Gene ID (NCBI): 571 ENSEMBL Gene ID: ENSG00000156273 RRID: AB_2882108 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O14867 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.