Proteintech, 66809-1-Ig, PABPC1,PABP Monoclonal antibody

Size: 20ul / 150ul The PABPC1,PABP (66809-1-Ig) by Proteintech is a Monoclonal antibody targeting PABPC1,PABP in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 66809-1-Ig targets PABPC1,PABP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, LNCaP cells, MCF-7 cells, T-47D cells, NCCIT cells Positive IHC detected in: mouse testis tissue, mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337]. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, hamster Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag27860 Product name: Recombinant human PABPC1,PABP protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 287-636 aa of BC015958 Sequence: RITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQRVANTSTQTMGPRPAAAAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV Predict reactive species Full Name: poly(A) binding protein, cytoplasmic 1 Calculated Molecular Weight: 71 kDa Observed Molecular Weight: 71 kDa GenBank Accession Number: BC015958 Gene Symbol: PABPC1 Gene ID (NCBI): 26986 RRID: AB_2882152 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P11940 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.