Proteintech, 66825-1-Ig, MYH14 Monoclonal antibody

Size: 20ul / 150ul The MYH14 (66825-1-Ig) by Proteintech is a Monoclonal antibody targeting MYH14 in WB, IHC, IF-P, ELISA applications with reactivity to Human, mouse samples 66825-1-Ig targets MYH14 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: COLO 320 cells, HT-29 cells Positive IHC detected in: human colon tissue, mouse small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse skeletal muscle tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:100000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4). Specification Tested Reactivity: Human, mouse Cited Reactivity: human, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28063 Product name: Recombinant human MYH14 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1231-1430 aa of NM_001077186 Sequence: MQARRGKGAWEKTRLALEAEVSELRAELSSLQTARQEGEQRRRRLELQLQEVQGRAGDGERARAEAAEKLQRAQAELENVSGALNEAESKTIRLSKELSSTEAQLHDAQELLQEETRAKLALGSRVRAMEAEAAGLREQLEEEAAARERAGRELQTAQAQLSEWRRRQEEEAGALEAGEEARRRAAREAEALTQRLAEKTE Predict reactive species Full Name: myosin, heavy chain 14 Calculated Molecular Weight: 228 kDa Observed Molecular Weight: 200-230 kDa GenBank Accession Number: NM_001077186 Gene Symbol: MYH14 Gene ID (NCBI): 79784 RRID: AB_2882168 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q7Z406 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.