Proteintech, 66850-1-Ig, KLF5 Monoclonal antibody

Size: 20ul / 150ul The KLF5 (66850-1-Ig) by Proteintech is a Monoclonal antibody targeting KLF5 in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 66850-1-Ig targets KLF5 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, A549 cells, NCI-H1299 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension Background Information KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag15403 Product name: Recombinant human KLF5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-350 aa of BC042131 Sequence: MATRVLSMSARLGPVPQPPAPQDEPVFAQLKPVLGAANPARDAALFPGEELKHAHHRPQAQPAPAQAPQPAQPPATGPRLPPEDLVQTRCEMEKYLTPQLPPVPIIPEHKKYRRDSASVVDQFFTDTEGLPYSINMNVFLPDITHLRTGLYKSQRPCVTHIKTEPVAIFSHQSETTAPPPAPTQALPEFTSIFSSHQTAAPEVNNIFIKQELPTPDLHLSVPTQQGHLYQLLNTPDLDMPSSTNQTAAMDTLNVSMSAAMAGLNTHTSAVPQTAVKQFQGMPPCTYTMPSQFLPQQATYFPPSPPSSEPGSPDRQAEMLQNLTPPPSYAATIASKLAIHNPNLPTTLPVN Predict reactive species Full Name: Kruppel-like factor 5 (intestinal) Calculated Molecular Weight: 457 aa, 51 kDa Observed Molecular Weight: 51-57 kDa GenBank Accession Number: BC042131 Gene Symbol: KLF5 Gene ID (NCBI): 688 RRID: AB_2882190 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q13887 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.