Proteintech, 66863-1-Ig, Phospho-Histone H3 (Ser10) Monoclonal antibody

Size: 20ul / 100ul The Phospho-Histone H3 (Ser10) (66863-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-Histone H3 (Ser10) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 66863-1-Ig targets Phospho-Histone H3 (Ser10) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells Positive IF/ICC detected in: C2C12 cells, HeLa cells, MCF-7 cells Positive FC (Intra) detected in: nocodazole treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:600-1:2400 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, chicken Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 1, H3a Calculated Molecular Weight: 15 kDa Observed Molecular Weight: 15-17 kDa GenBank Accession Number: NM_003529 Gene Symbol: HIST1H3A Gene ID (NCBI): 8350 RRID: AB_2882201 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P68431 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.