Proteintech, 66884-1-Ig, ASF1A Monoclonal antibody

Size: 20ul / 150ul The ASF1A (66884-1-Ig) by Proteintech is a Monoclonal antibody targeting ASF1A in WB, IF/ICC, ELISA applications with reactivity to human, rat samples 66884-1-Ig targets ASF1A in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: Jurkat cells, HSC-T6 cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit. Specification Tested Reactivity: human, rat Host / Isotype: Mouse / IgG3 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag1233 Product name: Recombinant human ASF1A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-204 aa of BC010878 Sequence: MAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRHMFVFQADAPNPGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNILASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM Predict reactive species Full Name: ASF1 anti-silencing function 1 homolog A (S. cerevisiae) Calculated Molecular Weight: 204 aa, 23 kDa Observed Molecular Weight: 21-23 kDa GenBank Accession Number: BC010878 Gene Symbol: ASF1A Gene ID (NCBI): 25842 RRID: AB_2882216 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9Y294 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.