Proteintech, 66888-1-Ig, mTOR Monoclonal antibody

Size: 20ul / 150ul The mTOR (66888-1-Ig) by Proteintech is a Monoclonal antibody targeting mTOR in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 66888-1-Ig targets mTOR in WB, IHC, IF/ICC, IP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: LNCaP cells, HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue, ROS1728 cells, Caco-2 cells, HepG2 cells, Jurkat cells, NCI-H1299 cells, THP-1 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells Positive IP detected in: HeLa cells Positive IHC detected in: human colon cancer tissue, human breast cancer tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, chicken, bovine, hamster, goat, zebra finches Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28395 Product name: Recombinant human MTOR protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 27-291 aa of NM_004958 Sequence: MSGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQ Predict reactive species Full Name: FK506 binding protein 12-rapamycin associated protein 1 Calculated Molecular Weight: 289 kDa Observed Molecular Weight: 250-289 kDa GenBank Accession Number: NM_004958 Gene Symbol: mTOR Gene ID (NCBI): 2475 RRID: AB_2882219 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P42345 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.