Proteintech, 66899-1-Ig, SPTLC1 Monoclonal antibody

Size: 20ul / 150ul The SPTLC1 (66899-1-Ig) by Proteintech is a Monoclonal antibody targeting SPTLC1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 66899-1-Ig targets SPTLC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, HEK-293 cells, RAW 264.7 cells, HT-29 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:490-1:1962 Background Information SPTLC1 is a subunit of serine palmitoyltransferase (SPT) which is the key enzyme in sphingolipid biosynthesis and is essential for embryogenesis and cell survival. Mutations in the SPTLC1 gene (C133W, C133Y, V144D, and G387A) were reported to be responsible for the development of an inherited sensory neuropathy (hereditary sensory neuropathy type I, HSN1). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7876 Product name: Recombinant human SPTLC1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-143 aa of BC007085 Sequence: MATATEQWVLVEMVQALYEAPAYHLILEGILILWIIRLLFSKTYKLQERSDLTVKEKEELIEEWQPEPLVPPVPKDHPALNYNIVSGPPSHKTVVNGKECINFASFNFLGLLDNPRVKAAALASLKKYGVGTCGPRGFYGTFE Predict reactive species Full Name: serine palmitoyltransferase, long chain base subunit 1 Calculated Molecular Weight: 53 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC007085 Gene Symbol: SPTLC1 Gene ID (NCBI): 10558 RRID: AB_2882228 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O15269 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.