Proteintech, 66910-1-Ig, INF2 Monoclonal antibody

Size: 20ul / 150ul The INF2 (66910-1-Ig) by Proteintech is a Monoclonal antibody targeting INF2 in WB, IHC, IF/ICC, ELISA applications with reactivity to Human samples 66910-1-Ig targets INF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: HeLa cells, A431 cells, PC-3 cells, HEK-293 cells, HepG2 cells Positive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information INF2 is a member of the formin family of actin-regulating proteins. INF2 is peripherally bound to the cytoplasmic face of the endoplasmic reticulum (ER). Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. The predicted MW of INF2 is around 136 kDa, while higher molecular mass from 140-200 kDa has been reported in literature (26383224,20493814,19366733). Specification Tested Reactivity: Human Cited Reactivity: mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17043 Product name: Recombinant human INF2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-350 aa of BC008756 Sequence: MVRPKAQLVLAACESLLTSRQLPIFCQLILRIGNFLNYGSHTGDADGFKISTLLKLTETKSQQNRVTLLHHVLEEAEKSHPDLLQLPRDLEQPSQAAGINLEIIRSEASSNLKKLLETERKVSASVAEVQEQYTERLQASISAFRALDELFEAIEQKQRELADYLCEDAQQLSLEDTFSTMKAFRDLFLRALKENKDRKEQAAKAERRKQQLAEEEARRPRGEDGKPVRKGPGKQEEVCVIDALLADIRKGFQLRKTARGRGDTDGGSKAASMDPPRATEPVATSNPAGDPVGSTRCPASEPGLDATTASESRGWDLVDAVTPGPQPTLEQLEEGGPRPLERRSSWYVDA Predict reactive species Full Name: inverted formin, FH2 and WH2 domain containing Calculated Molecular Weight: 1249 aa, 136 kDa Observed Molecular Weight: 150-170 kDa GenBank Accession Number: BC008756 Gene Symbol: INF2 Gene ID (NCBI): 64423 RRID: AB_2882237 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q27J81 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.