Proteintech, 66977-1-Ig, CAPN2 Monoclonal antibody

Size: 20ul / 150ul The CAPN2 (66977-1-Ig) by Proteintech is a Monoclonal antibody targeting CAPN2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 66977-1-Ig targets CAPN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, human placenta tissue, U2OS cells, LNCaP cells, HEK-293 cells, rat brain tissue, mouse brain tissue, HepG2 cells Positive IHC detected in: human pancreas cancer tissue, human stomach cancer tissue, human colon cancer tissue, rat colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928, and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from the human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease that catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28427 Product name: Recombinant human CAPN2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-330 aa of BC021303 Sequence: MAGIAAKLAKDREAAEGLGSHERAIKYLNQDYEALRNECLEAGTLFQDPSFPAIPSALGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELKKPPPNLFKIIQKALQKGSLLGCSIDITSAADSEAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPWGEVEWTGRWNDNCPSWNTIDPEERERLTRRHEDGEFWMSFSD Predict reactive species Full Name: calpain 2, (m/II) large subunit Calculated Molecular Weight: 700 aa, 80 kDa Observed Molecular Weight: 72-80 kDa GenBank Accession Number: BC021303 Gene Symbol: CAPN2 Gene ID (NCBI): 824 RRID: AB_2882297 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P17655 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.