Proteintech, 66998-1-Ig, RAG2 Monoclonal antibody

Size: 20ul / 150ul The RAG2 (66998-1-Ig) by Proteintech is a Monoclonal antibody targeting RAG2 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, pig samples 66998-1-Ig targets RAG2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, pig samples. Tested Applications Positive WB detected in: Jurkat cells, Raji cells, Ramos cells Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3 Specification Tested Reactivity: human, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag16780 Product name: Recombinant human RAG2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 175-527 aa of BC022397 Sequence: VADCLPCVFLVDFEFGCATSYILPELQDGLSFHVSIAKNDTIYILGGHSLANNIRPANLYRIRVDLPLGSPAVNCTVLPGGISVSSAILTQTNNDEFVIVGGYQLENQKRMICNIISLEDNKIEIREMETPDWTPDIKHSKIWFGSNTGNGTVFLGIPGDNKQVVSEGFYFYMLKCAEDDTNEEQTTFTNSQTSTEDPGDSTPFEDSEEFCFSAEANSFDGDDEFDTYNEDDEEDESETGYWITCCPTCDVDINTWVPFYSTELNKPAMIYCSHGDGHWVHAQCMDLAERTLIHLSAGSNKYYCNEHVEIARALHTPQRVLPLKKPPMKSLRKKGSGKILTPAKKSFLRRLFD Predict reactive species Full Name: recombination activating gene 2 Calculated Molecular Weight: 527 aa, 59 kDa Observed Molecular Weight: 57-62 kDa GenBank Accession Number: BC022397 Gene Symbol: RAG2 Gene ID (NCBI): 5897 RRID: AB_2882315 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P55895 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.