Proteintech, 67021-1-Ig, NFS1 Monoclonal antibody

Size: 20ul / 150ul The NFS1 (67021-1-Ig) by Proteintech is a Monoclonal antibody targeting NFS1 in WB, ELISA applications with reactivity to human, mouse, rat samples 67021-1-Ig targets NFS1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HSC-T6 cells, HepG2 cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8017 Product name: Recombinant human NFS1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-189 aa of BC018471 Sequence: MLLRAAWRRAAVAVTAAPGPKPAAPTRGLRLRVGDRAPQSAVPADTAAAPEVGPVLRPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKTESHSVIQAGVQWHDLDSLQAPPPGFTPFSCLSLLSSWDYRRPPPHPANFLYF Predict reactive species Full Name: NFS1 nitrogen fixation 1 homolog (S. cerevisiae) Calculated Molecular Weight: 50 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC018471 Gene Symbol: NFS1 Gene ID (NCBI): 9054 RRID: AB_2918480 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9Y697 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.