Proteintech, 67045-1-Ig, CYP27A1 Monoclonal antibody

Size: 20ul / 150ul CYP27A1 Monoclonal Antibody for WB, IHC, IF-P, ELISA 67045-1-Ig targets CYP27A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Caco-2 cells, HepG2 cells, HT-29 cells, rat liver tissue Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human liver cancer tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7746 Product name: Recombinant human CYP27A1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 183-531 aa of BC051851 Sequence: DDFMTRLDQLRAESASGNQVSDMAQLFYYFALEAICYILFEKRIGCLQRSIPEDTVTFVRSIGLMFQNSLYATFLPKWTRPVLPFWKRYLDGWNAIFSFGKKLIDEKLEDMEAQLQAAGPDGIQVSGYLHFLLASGQLSPREAMGSLPELLMAGVDTTSNTLTWALYHLSKDPEIQEALHEEVVGVVPAGQVPQHKDFAHMPLLKAVLKETLRLYPVVPTNSRIIEKEIEVDGFLFPKNTQFVFCHYVVSRDPTAFSEPESFQPHRWLRNSQPATPRIQHPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPETGELKSVARIVLVPNKKVGLQFLQRQC Predict reactive species Full Name: cytochrome P450, family 27, subfamily A, polypeptide 1 Calculated Molecular Weight: 60 kDa Observed Molecular Weight: 54-60 kDa GenBank Accession Number: BC051851 Gene Symbol: CYP27A1 Gene ID (NCBI): 1593 RRID: AB_2882359 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q02318 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.