Proteintech, 67062-1-Ig, PDE4D Monoclonal antibody

Size: 20ul / 150ul The PDE4D (67062-1-Ig) by Proteintech is a Monoclonal antibody targeting PDE4D in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 67062-1-Ig targets PDE4D in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: A549 cells, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, pig brain tissue, mouse cerebellum tissue Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:3000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:800-1:3200 Background Information PDE4D(cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also known as DPDE3, PDE43 and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram (PMID:16730340). It has some isoforms and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in the hippocampus from a patient with advanced Alzheimer disease (PMID:17353396). Bands of 90-110 kDa correspond to the long PDE4D isoforms, and bands of 66-84 kDa are the short PDE4D isoforms (PMID: 17215491). Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8302 Product name: Recombinant human PDE4D protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 170-518 aa of BC036319 Sequence: YHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVFNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACVIDDRSPDT Predict reactive species Full Name: phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) Calculated Molecular Weight: 809 aa, 91 kDa Observed Molecular Weight: 91-110 kDa, 66-84 kDa GenBank Accession Number: BC036319 Gene Symbol: PDE4D Gene ID (NCBI): 5144 RRID: AB_2882372 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q08499 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.