Proteintech, 67066-1-Ig, ATF5 Monoclonal antibody

Size: 20ul / 150ul The ATF5 (67066-1-Ig) by Proteintech is a Monoclonal antibody targeting ATF5 in WB, ELISA applications with reactivity to Human samples 67066-1-Ig targets ATF5 in WB, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: Jurkat cells, HeLa cells, HEK-293 cells, SH-SY5Y cells, A431 cells, HT-29 cells, MCF-7 cells, MDA-MB-231 cells, SK-BR-3 cells, T-47D cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Background Information ATF5, also named as Activating transcription factor 5, is a 282 amino acid protein, which belongs to the bZIP family. ATF5 is Widely expressed with higher expression levels in liver. ATF5 is actively transported to the centrosome and accumulated in the pericentriolar material (PCM) during G1 to M phase via a microtubule-dependent mechanism. During late telophase and cytokinesis, it translocates from the centrosome to the midbody (PMID: 26213385) . ATF5 as a transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically ATF5 is involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631). Specification Tested Reactivity: Human Cited Reactivity: human, mouse, chicken Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7214 Product name: Recombinant human ATF5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-282 aa of BC005174 Sequence: MSLLATLGLELDRALLPASGLGWLVDYGKLPPAPAPLAPYEVLGGALEGGLPVGGEPLAGDGFSDWMTERVDFTALLPLEPPLPPGTLPQPSPTPPDLEAMASLLKKELEQMEDFFLDAPLLPPPSPPPLPPPPLPPAPSLPLSLPSFDLPQPPVLDTLDLLAIYCRNEAGQEEVGMPPLPPPQQPPPPSPPQPSRLAPYPHPATTRGDRKQKKRDQNKSAALRYRQRKRAEGEALEGECQGLEARNRELKERAESVEREIQYVKDLLIEVYKARSQRTRSC Predict reactive species Full Name: activating transcription factor 5 Calculated Molecular Weight: 31 kDa Observed Molecular Weight: 30-35 kDa GenBank Accession Number: BC005174 Gene Symbol: ATF5 Gene ID (NCBI): 22809 RRID: AB_2882376 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9Y2D1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.