Proteintech, 67072-1-Ig, ASK1 Monoclonal antibody

Size: 20ul / 150ul The ASK1 (67072-1-Ig) by Proteintech is a Monoclonal antibody targeting ASK1 in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, Mouse, Rat samples 67072-1-Ig targets ASK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples. Tested Applications Positive WB detected in: HSC-T6 cells, HEK-293 cells, Jurkat cells, HeLa cells, MCF-7 cells, NIH/3T3 cells Positive IHC detected in: human pancreas cancer tissue, mouse heart tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:150-1:600 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 67072-1-Ig antibody detects the degraded product of ASK1 in SDS-PAGE. Specification Tested Reactivity: Human, Mouse, Rat Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag5818 Product name: Recombinant human ASK1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 681-936 aa of BC054503 Sequence: GDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDE Predict reactive species Full Name: mitogen-activated protein kinase kinase kinase 5 Calculated Molecular Weight: 155 kDa Observed Molecular Weight: 110 kDa GenBank Accession Number: BC054503 Gene Symbol: ASK1 Gene ID (NCBI): 4217 RRID: AB_2882381 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q99683 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.