Proteintech, 67115-1-Ig, L1CAM/CD171 Monoclonal antibody

Size: 20ul / 150ul The L1CAM/CD171 (67115-1-Ig) by Proteintech is a Monoclonal antibody targeting L1CAM/CD171 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 67115-1-Ig targets L1CAM/CD171 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: pig brain tissue, pig cerebellum tissue, Rabbit brain tissue, rat brain tissue, mouse brain tissue Positive IHC detected in: human colon cancer tissue, human brain tissue, human colon tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503). Specification Tested Reactivity: human, mouse, rat, pig, rabbit Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17706 Product name: Recombinant human L1CAM protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 794-1143 aa of BC126229 Sequence: QGKGPEPQVTIGYSGEDYPQAIPELEGIEILNSSAVLVKWRPVDLAQVKGHLRGYNVTYWREGSQRKHSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNGRGSGPASEFTFSTPEGVPGHPEALHLECQSNTSLLLRWQPPLSHNGVLTGYVLSYHPLDEGGKGQLSFNLRDPELRTHNLTDLSPHLRYRFQLQATTKEGPGEAIVREGGTMALSGISDFGNISATAGENYSVVSWVPKEGQCNFRFHILFKALGEEKGGASLSPQYVSYNQSSYTQWDLQPDTDYEIHLFKERMFRHQMAVKTNGTGRVRLPPAGFATEGWFIGFVSAIILLLLVLLILCFI Predict reactive species Full Name: L1 cell adhesion molecule Calculated Molecular Weight: 1257 aa, 140 kDa Observed Molecular Weight: 220 kDa GenBank Accession Number: BC126229 Gene Symbol: L1CAM Gene ID (NCBI): 3897 RRID: AB_2882419 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P32004 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.