Proteintech, 67154-1-Ig, SUMO2/3 Monoclonal antibody

Size: 20ul / 150ul The SUMO2/3 (67154-1-Ig) by Proteintech is a Monoclonal antibody targeting SUMO2/3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 67154-1-Ig targets SUMO2/3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, Jurkat cells, A549 cells, NIH/3T3 cells, K-562 cells, HSC-T6 cells, PC-12 cells Positive IHC detected in: human prostate cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28672 Product name: Recombinant human SUMO2/3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-95 aa of BC008450 Sequence: MADEKPKEGVKTENNNHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGGVY Predict reactive species Full Name: SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) Calculated Molecular Weight: 11 kDa Observed Molecular Weight: 18 kDa GenBank Accession Number: BC008450 Gene Symbol: SUMO2 Gene ID (NCBI): 6613 RRID: AB_2882451 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P61956 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.