Proteintech, 67158-1-Ig, PLVAP Monoclonal antibody

Size: 20ul / 150ul The PLVAP (67158-1-Ig) by Proteintech is a Monoclonal antibody targeting PLVAP in WB, IHC, IF-Fro, ELISA applications with reactivity to human, mouse, pig samples 67158-1-Ig targets PLVAP in WB, IHC, IF-Fro, ELISA applications and shows reactivity with human, mouse, pig samples. Tested Applications Positive WB detected in: human placenta tissue, pig spleen tissue, pig kidney tissue Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-Fro detected in: mouse small intestine tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800 Background Information Plasmalemma vesicle-associated protein (PLVAP) is an endothelial-associated protein that is involved in endothelial diaphragm formation and maintenance of basal vascular permeability. At the same time, PLVAP has roles in immunity by facilitating leukocyte diapedesis at inflammatory sites and controlling peripheral lymph node morphogenesis and the entry into soluble ags into lymph node conduits .(PMID: 36884119, PMID: 37810232) Specification Tested Reactivity: human, mouse, pig Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6359 Product name: Recombinant human PLVAP protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 63-442 aa of BC056414 Sequence: TERRAEGLYSQLLGLTASQSNLTKELNFTTRAKDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCDALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLAKEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEELARSLRADIERVARENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREAKLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSQPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSG Predict reactive species Full Name: plasmalemma vesicle associated protein Calculated Molecular Weight: 51 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC056414 Gene Symbol: PLVAP Gene ID (NCBI): 83483 RRID: AB_2882455 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9BX97 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.