Proteintech, 67161-1-Ig, GBP1 Monoclonal antibody

Size: 20ul / 150ul The GBP1 (67161-1-Ig) by Proteintech is a Monoclonal antibody targeting GBP1 in WB, IHC, ELISA applications with reactivity to human, rat, pig samples 67161-1-Ig targets GBP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, pig samples. Tested Applications Positive WB detected in: HeLa cells, human placenta tissue, HUVEC cells, pig spleen tissue, rat spleen tissue, HEK-293 cells Positive IHC detected in: human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Background Information Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by IFN and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP. GBP1 is induced in response to type I and type II IFNs and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica. Specification Tested Reactivity: human, rat, pig Cited Reactivity: human, mouse, chinese tree shrews Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7767 Product name: Recombinant human GBP1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 242-592 aa of BC002666 Sequence: VHRRKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIQVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVQKAIAHYEQQMGQKVQLPTESLQELLDLHRDSEREAIEVFIRSSFKDVDHLFQKELAAQLEKKRDDFCKQNQEASSDRCSGLLQVIFSPLEEEVKAGIYSKPGGYRLFVQKLQDLKKKYYEEPRKGIQAEEILQTYLKSKESMTDAILQTDQTLTEKEKEIEVERVKAESAQASAKMLQEMQRKNEQMMEQKERSYQEHLKQLTEKMENDRVQLLKEQERTLALKLQEQEQLLKEGFQKESRIMKNEIQDLQTKMRRRKACTIS Predict reactive species Full Name: GTP binding protein 1 Calculated Molecular Weight: 68 kDa Observed Molecular Weight: 68 kDa GenBank Accession Number: BC002666 Gene Symbol: GBP1 Gene ID (NCBI): 2633 RRID: AB_2882457 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P32455 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.