Proteintech, 67265-1-Ig, PLXNB2 Monoclonal antibody

Size: 20ul / 150ul The PLXNB2 (67265-1-Ig) by Proteintech is a Monoclonal antibody targeting PLXNB2 in WB, IHC, ELISA applications with reactivity to Human, Mouse, Rat samples 67265-1-Ig targets PLXNB2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples. Tested Applications Positive WB detected in: MDA-MB-231 cells, rat brain tissue, ATDC-5 cells, U2OS cells, Mouse brain tissue, MCF-7 cells, A549 cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 20-466aa of human plexin B2 can recognize precursor and alpha subunit of plexin B2. Specification Tested Reactivity: Human, Mouse, Rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28706 Product name: Recombinant human PLXNB2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 20-466 aa of NM_012401 Sequence: LRPRKLDFFRSEKELNHLAVDEASGVVYLGAVNALYQLDAKLQLEQQVATGPALDNKKCTPPIEASQCHEAEMTDNVNQLLLLDPPRKRLVECGSLFKGICALRALSNISLRLFYEDGSGEKSFVASNDEGVATVGLVSSTGPGGDRVLFVGKGNGPHDNGIIVSTRLLDRTDSREAFEAYTDHATYKAGYLSTNTQQFVAAFEDGPYVFFVFNQQDKHPARNRTLLARMCREDPNYYSYLEMDLQCRDPDIHAAAFGTCLAASVAAPGSGRVLYAVFSRDSRSSGGPGAGLCLFPLDKVHAKMEANRNACYTGTREARDIFYKPFHGDIQCGGHAPGSSKSFPCGSEHLPYPLGSRDGLRGTAVLQRGGLNLTAVTVAAENNHTVAFLGTSDGRILKVYLTPDGTSSEYDSILVEINKRVKRDLVLSGDLGSLYAMTQDKVFRLPV Predict reactive species Full Name: plexin B2 Calculated Molecular Weight: 205 kDa Observed Molecular Weight: 240 kDa GenBank Accession Number: NM_012401 Gene Symbol: PLXNB2 Gene ID (NCBI): 23654 RRID: AB_2882536 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O15031 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.