Proteintech, 67280-1-Ig, SNRPN/SNRPB Monoclonal antibody

Size: 20ul / 150ul The SNRPN/SNRPB (67280-1-Ig) by Proteintech is a Monoclonal antibody targeting SNRPN/SNRPB in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 67280-1-Ig targets SNRPN/SNRPB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 Background Information SNRPN, or SNURF-SNRPN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide.The SNRPN gene is transcribed exclusively from the paternally inherited chromosome and shows highest expression in brain and heart. SNRPN may play a role in etiology of Prader-Willi syndrome. This antibody recognizes SNRPN and SNRPB due to high amino acid sequence homology. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag14030 Product name: Recombinant human SNRPN protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-240 aa of BC000611 Sequence: MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNAKQPEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGVGRAAGRGVPAGVPIPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAVAATASIAGAPTQYPPGRGTPPPPVGRATPPPGIMAPPPGMRPPMGPPIGLPPARGTPIGMPPPGMRPPPPGIRGPPPPGMRPPRP Predict reactive species Full Name: small nuclear ribonucleoprotein polypeptide N Calculated Molecular Weight: 240 aa, 25 kDa Observed Molecular Weight: 27-29 kDa GenBank Accession Number: BC000611 Gene Symbol: SNRPN Gene ID (NCBI): 6638 RRID: AB_2882548 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P63162 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.