Proteintech, 67288-1-Ig, Collagen Type I Monoclonal antibody

Size: 20ul / 150ul The Collagen Type I (67288-1-Ig) by Proteintech is a Monoclonal antibody targeting Collagen Type I in WB, IHC, IF/ICC, IF-P, ELISA applications with reactivity to human, pig samples 67288-1-Ig targets Collagen Type I in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human, pig samples. Tested Applications Positive WB detected in: pig colon tissue, human cervical cancer tissue, human placenta tissue, MG-63 cells, pig lung tissue, pig skin tissue Positive IHC detected in: human breast cancer tissue, human colon tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human colon cancer tissue, Human Fibroblasts Positive IF/ICC detected in: U-251 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:2500-1:10000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727). It is reported that the molecular weight is 200kDa(PMID: 28526934). Specification Tested Reactivity: human, pig Cited Reactivity: human, pig, rabbit, zebrafish Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: collagen, type I, alpha 1 Calculated Molecular Weight: 139 kDa Observed Molecular Weight: 100-140 kDa，200kDa GenBank Accession Number: NM_000088 Gene Symbol: COL1A1 Gene ID (NCBI): 1277 RRID: AB_2882554 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P02452 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.