Proteintech, 67311-1-Ig, DCPS Monoclonal antibody

Size: 20ul / 150ul The DCPS (67311-1-Ig) by Proteintech is a Monoclonal antibody targeting DCPS in WB, IHC, IF-P, ELISA applications with reactivity to Human, Mouse, Rat samples 67311-1-Ig targets DCPS in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples. Tested Applications Positive WB detected in: HeLa cells, rat spleen tissue, mouse brain tissue, HepG2 cells, K-562 cells, Jurkat cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human liver cancer tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information DcpS, which is a member of the histidine triad (HIT) superfamily of pyrophosphatases, is the first HIT protein with a defined biological function and identifies the HIT motif as a new mRNA decapping domain. DcpS can encode a 38-40 kDa protein, and has the potential to form a homodimer of 80 kDa(PMID: 12198172). Specification Tested Reactivity: Human, Mouse, Rat Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8872 Product name: Recombinant human DCPS protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-337 aa of BC014532 Sequence: MADAAPQLGKRKRELDVEEAHAASTEEKEAGVGNGTCAPVRLPFSGFRLQKVLRESARDKIIFLHGKVNEASEDGDGEDAVVILEKTPFQVEQVAQLLTGSPELQLQFSNDIYSTYHLFPPRQLNDVKTTVVYPATEKHLQKYLRQDLRLIRETGDDYRNITLPHLESQSLSIQWVYNILDKKAEADRIVFENPDPSDGFVLIPDLKWNQQQLDDLYLIAICHRRGIRSLRDLTPEHLPLLRNILHQGQEAILQRYRMKGDHLRVYLHYLPSYYHLHVHFTALGFEAPGSGVERAHLLAEVIENLECDPRHYQQRTLTFALRADDPLLKLLQEAQQS Predict reactive species Full Name: decapping enzyme, scavenger Calculated Molecular Weight: 337 aa, 39 kDa Observed Molecular Weight: 35-40 kDa GenBank Accession Number: BC014532 Gene Symbol: DCPS Gene ID (NCBI): 28960 RRID: AB_2882571 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q96C86 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.